Exotic diseases of dogs and cats at risk of importation to Ireland

Changes in legislation that facilitate movement of companion animals within the European Union will expose those animals to microbial and parasitic organisms currently exotic to Ireland. This paper reviewed information on the exotic diseases most likely to be introduced to Ireland by travelling dogs and cats: rabies, leishmaniosis, babesiosis, ehrlichiosis, anaplasmosis and dirofilariosis.</p

Studies of the Links Between Quasar Black Hole Accretion and Star Formation in Their Host Galaxies: Data Analysis of the Herschel Astrophysical Terahertz Large Area Survey

The topic of this thesis is to gather evidence for a link between star formation in galaxies that contain a quasar in their nuclei. For quasars I argue that the best technique to measure the star formation rates is to use the far infrared. I used the Herschel PACS 100 and 160 micron infrared data from the Herschel ATLAS programme to examine star formation rates in quasar host galaxies. I performed a stacking analysis of the PACS 100 and 160 micron data of a sample of 411 quasar candidates from the SDSS and 2SLAQ catalogues. The quasars are detected at up to 32σ, which I infer is from star formation in the galaxies that contain these quasars. In order to obtain this data I have written a new timeline filtering subprogramme, plus a cross-scan filtering subprogramme, for the PACS data analysis pipeline. The outputs of this programme, using the early science phase Herschel data, were used to do the quasar stacking analysis. I was also able to derive the formal minimuin-variance point source filter using the calculus of variations with undertermined Langrangian multiplers. This kernel optimises the signal to noise but leads to false positive signals. I opted to use a modified kernel that whitens the power spectrum of the noise, and gives us a more reliable point source detection. However, the SNR penalty, for different PACS pixels and data sets, lies in the range 30% to 40%

Imported disease of dogs and cats exotic to Ireland: Echinococcus multilocularis

Changes in legislation that facilitate the movement of animals within the European Union may increase the risk that some microbial and parasitic organisms, currently exotic to Ireland, will be introduced by travelled pet animals. It is possible that the fox tapeworm, Echinococcus multilocularis, might be introduced in that manner from any of the several member states in which it is endemic. Red foxes are the principal definitive hosts of E. multilocularis but dogs and cats can also be infected. Infection in the definitive host is of little clinical significance, but aberrant infection of humans results in alveolar echinococcosis, a debilitating disease that has a high mortality rate. Humans acquire the organism by ingestion of Echinococcus multilocularis eggs excreted by definitive hosts; the larval metacestodes develop primarily in the liver, in the initial asymptomatic phase as small, well-encapsulated cysts. Over time, perhaps five to 15 years, progressive local infiltration and secondary cyst development at distant sites occur with resultant clinical signs. Patients with infiltrative liver disease present with cholestatic jaundice, epigastric pain, fatigue, weight loss and hepatomegaly. If left untreated, the disease can be fatal

Development of an acoustic communication link for micro underwater vehicles

PhD ThesisIn recent years there has been an increasing trend towards the use of Micro Remotely Operated Vehicles (μROVs), such as the Videoray and Seabotix LBV products, for a range of subsea applications, including environmental monitoring, harbour security, military surveillance and offshore inspection. A major operational limitation is the umbilical cable, which is traditionally used to supply power and communications to the vehicle. This tether has often been found to significantly restrict the agility of the vehicle or in extreme cases, result in entanglement with subsea structures. This thesis addresses the challenges associated with developing a reliable full-duplex wireless communications link aimed at tetherless operation of a μROV. Previous research has demonstrated the ability to support highly compressed video transmissions over several kilometres through shallow water channels with large range-depth ratios. However, the physical constraints of these platforms paired with the system cost requirements pose significant additional challenges. Firstly, the physical size/weight of transducers for the LF (8-16kHz) and MF (16-32kHz) bands would significantly affect the dynamics of the vehicle measuring less than 0.5m long. Therefore, this thesis explores the challenges associated with moving the operating frequency up to around 50kHz centre, along with the opportunities for increased data rate and tracking due to higher bandwidth. The typical operating radius of μROVs is less than 200m, in water < 100m deep, which gives rise to multipath channels characterised by long timespread and relatively sparse arrivals. Hence, the system must be optimised for performance in these conditions. The hardware costs of large multi-element receiver arrays are prohibitive when compared to the cost of the μROV platform. Additionally, the physical size of such arrays complicates deployment from small surface vessels. Although some recent developments in iterative equalisation and decoding structures have enhanced the performance of single element receivers, they are not found to be adequate in such channels. This work explores the optimum cost/performance trade-off in a combination of a micro beamforming array using a Bit Interleaved Coded Modulation with Iterative Decoding (BICM-ID) receiver structure. The highly dynamic nature of μROVs, with rapid acceleration/deceleration and complex thruster/wake effects, are also a significant challenge to reliable continuous communications. The thesis also explores how these effects can best be mitigated via advanced Doppler correction techniques, and adaptive coding and modulation via a simultaneous frequency multiplexed down link. In order to fully explore continuous adaptation of the transmitted signals, a real-time full-duplex communication system was constructed in hardware, utilising low cost components and a highly optimised PC based receiver structure. Rigorous testing, both in laboratory conditions and through extensive field trials, have enabled the author to explore the performance of the communication link on a vehicle carrying out typical operations and presenting a wide range of channel, noise, Doppler and transmission latency conditions. This has led to a comprehensive set of design recommendations for a reliable and cost effective link capable of continuous throughputs of >30 kbits/s

A critical role for network structure in seizure onset: a computational modeling approach

Published onlineJournal ArticleRecent clinical work has implicated network structure as critically important in the initiation of seizures in people with idiopathic generalized epilepsies. In line with this idea, functional networks derived from the electroencephalogram (EEG) at rest have been shown to be significantly different in people with generalized epilepsy compared to controls. In particular, the mean node degree of networks from the epilepsy cohort was found to be statistically significantly higher than those of controls. However, the mechanisms by which these network differences can support recurrent transitions into seizures remain unclear. In this study, we use a computational model of the transition into seizure dynamics to explore the dynamic consequences of these differences in functional networks. We demonstrate that networks with higher mean node degree are more prone to generating seizure dynamics in the model and therefore suggest a mechanism by which increased mean node degree of brain networks can cause heightened ictogenicity.Medical Research Counci

Electrical and network neuronal properties are preferentially disrupted in dorsal, but not ventral, medial entorhinal cortex in a mouse model of Tauopathy

The entorhinal cortex (EC) is one of the first areas to be disrupted in neurodegenerative diseases such as Alzheimer's disease and frontotemporal dementia. The responsiveness of individual neurons to electrical and environmental stimuli varies along the dorsal-ventral axis of the medial EC (mEC) in a manner that suggests this topographical organization plays a key role in neural encoding of geometric space. We examined the cellular properties of layer II mEC stellate neurons (mEC-SCs) in rTg4510 mice, a rodent model of neurodegeneration. Dorsoventral gradients in certain intrinsic membrane properties, such as membrane capacitance and afterhyperpolarizations, were flattened in rTg4510 mEC-SCs, while other cellular gradients [e.g., input resistance (Ri), action potential properties] remained intact. Specifically, the intrinsic properties of rTg4510 mEC-SCs in dorsal aspects of the mEC were preferentially affected, such that action potential firing patterns in dorsal mEC-SCs were altered, while those in ventral mEC-SCs were unaffected. We also found that neuronal oscillations in the gamma frequency band (30-80 Hz) were preferentially disrupted in the dorsal mEC of rTg4510 slices, while those in ventral regions were comparatively preserved. These alterations corresponded to a flattened dorsoventral gradient in theta-gamma cross-frequency coupling of local field potentials recorded from the mEC of freely moving rTg4510 mice. These differences were not paralleled by changes to the dorsoventral gradient in parvalbumin staining or neurodegeneration. We propose that the selective disruption to dorsal mECs, and the resultant flattening of certain dorsoventral gradients, may contribute to disturbances in spatial information processing observed in this model of dementia. SIGNIFICANCE STATEMENT: The medial entorhinal cortex (mEC) plays a key role in spatial memory and is one of the first areas to express the pathological features of dementia. Neurons of the mEC are anatomically arranged to express functional dorsoventral gradients in a variety of neuronal properties, including grid cell firing field spacing, which is thought to encode geometric scale. We have investigated the effects of tau pathology on functional dorsoventral gradients in the mEC. Using electrophysiological approaches, we have shown that, in a transgenic mouse model of dementia, the functional properties of the dorsal mEC are preferentially disrupted, resulting in a flattening of some dorsoventral gradients. Our data suggest that neural signals arising in the mEC will have a reduced spatial content in dementia

Unrecognised Outbreak: Human parainfluenza virus infections in a pediatric oncology unit. A new diagnostic PCR and virus monitoring system may allow early detection of future outbreaks.

Background: Human parainfluenza viruses (HPIVs) are significant causes of both upper and lower respiratory tract infections with type 3 (HPIV3) causing the most severe disease in the immunocompromised cohorts.  The objective of this study was to analyse the epidemiological nature of a cluster of cases of HPIV3 in a pediatric oncology unit of a major teaching hospital. Methods: In order to determine whether the activity observed represented a deviation from the norm, seasonal trends of HPIV3 in the surrounding geographical area as well as on the ward in question were analysed.  The genetic link between cases was established by the phylogenetic analysis of the non-coding hypervariable region between the M (Matrix) and F (fusion) genes of HPIV3. The 15 cases involved and 15 unrelated cases were sequenced.  Transmission routes were subsequently inferred and visualized using Konstanz Information Miner (KNIME) 3.3.2. Results: Of the 15 cases identified, 14 were attributed to a point source outbreak. Two out of 14 outbreak cases were found to differ by a single mutation A182C. The outbreak strain was also seen in 1 out of 15 unrelated cases, indicating that it was introduced from the community. Transmission modeling was not able to link all the cases and establish a conclusive chain of transmission. No staff were tested during the outbreak period. No deaths occurred as a result of the outbreak. Conclusion: A point source outbreak of HPIV3 was recognized post factum on an oncology pediatric unit in a major teaching hospital. This raised concern about the possibility of a future more serious outbreak. Weaknesses in existing systems were identified and a new dedicated respiratory virus monitoring system introduced.  Pediatric oncology units require sophisticated systems for early identification of potentially life-threatening viral outbreaks

Controversies on the network theory of epilepsy : Debates held during the ICTALS 2019 conference

Acknowledgements We would like to acknowledge the contributions of the discussants to the exposition and discussion of the six debate topics. The discussants for debates 1-6 were Fabrice Wendling, Mark Cook, Mark Richardson, Thorsten Rings, Klaus Lehnertz and Piotr Suffczynski, respectively. Funding for ICTALS 2019 was received from the following foundations and industry partners: UCB S.A. (Belgium), American Epilepsy Society (AES), Epilepsy Innovation Institute (Ei2) and Epilepsy Foundation of America (EFA), NeuraLynx (Bozeman, MT, USA) and LivaNova (London, UK). The contribution of HZ was supported by award R01NS109062 from the National Institutes of Health, MG by the EPSRC via grants EP/P021417/1 and EP/N014391/1 and a Wellcome Trust Institutional Strategic Support Award (WT105618MA), and PJ by awards from the Ministry of Health of the Czech Republic AZV 17-28427A and the Czech Science Foundation 20-25298S. The opinions expressed in this article do not necessarily reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government.Peer reviewedPostprin

Adhesive and conformational behaviour of mycolic acid monolayers

We have studied the pH-dependent interaction between mycolic acid (MA) monolayers and hydrophobic and hydrophilic surfaces using molecular (colloidal probe) force spectroscopy. In both cases, hydrophobic and hydrophilic monolayers (prepared by Langmuir-Blodgett and Langmuir-Schaefer deposition on silicon or hydrophobized silicon substrates, respectively) were studied. The force spectroscopy data, fitted with classical DLVO (Derjaguin, Landau, Verwey, and Overbeek) theory to examine the contribution of electrostatic and van der Waals forces, revealed that electrostatic forces are the dominant contribution to the repulsive force between the approaching colloidal probe and MA monolayers. The good agreement between data and the DLVO model suggest that beyond a few nm away from the surface, hydrophobic, hydration, and specific chemical bonding are unlikely to contribute to any significant extent to the interaction energy between the probe and the surface. The pH-dependent conformation of MA molecules in the monolayer at the solid-liquid interface was studied by ellipsometry, neutron reflectometry, and with a quartz crystal microbalance. Monolayers prepared by the Langmuir-Blodgett method demonstrated a distinct pH-responsive behaviour, while monolayers prepared by the Langmuir-Schaefer method were less sensitive to pH variation. It was found that the attachment of water molecules plays a vital role in determining the conformation of the MA monolayers. (C) 2010 Elsevier B.V. All rights reserved